منابع مشابه
Karyotyping human and mouse cells using probes from single-sorted chromosomes and open source software.
Multispectral karyotyping analyzes all chromosomes in a single cell by labeling them with chromosome-specific probes conjugated to unique combinations of fluorophores. Currently available multispectral karyotyping systems require the purchase of specialized equipment and reagents. However, conventional laser scanning confocal microscopes that are capable of separating multiple overlapping emiss...
متن کاملDifferentiation of Mouse Embryonic Stem Cells into Hematopoietic Cells
Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...
متن کاملA Study of Cytogenetic Stability of Induced Pluripotent Stem Cells Using Karyotyping and Comet Assay Techniques
Background & Aims: Induced pluripotent stem cells (iPSCs) have the capability to undergo unlimited selfrenewal and differentiation into all cell types in the body. These cells are artificially derived from a nonpluripotent cell, typically human dermal fibroblasts (HDFs). The study of cytogenetic stability of these cells, in order to use iPS cells and apply studies in therapeutic applications, i...
متن کاملAutomated Karyotyping of Metaphase Cells with Touching Chromosomes
Since the birth of the automated karyotyping systems by the aid of computers, building a fully automated chromosome analysis system has been an ultimate goal. Along with many other challenges, automating chromosome classification and segmentation has been a major challenge especially due to overlapping and touching chromosomes. The earlier reported methods have limited success as they are sensi...
متن کاملSpectral karyotyping (SKY)
Published: 13/10/2010 Received: 02/02/2010 ecancer 2010, 4:181 DOI: 10.3332/ecancer.2010.181 Copyright: © the authors; licensee ecancermedicalscience. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the origin...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2008
ISSN: 1559-6095
DOI: 10.1101/pdb.prot4706